Industrial Microbiology

Macromolecule purification is critical in many life science workflows. However, resin-based ion exchange or affinity chromatography methods typically require sophisticated equipment and long set-up times, exhibit low flow rates, and suffer from limited yields. The small pore sizes and large bed volumes of conventional columns contribute to size exclusion and dilution effects, which limit the ability to purify larger macromolecules, demand further process steps to concentrate dilute eluates and increase reagent consumption. Furthermore, these purification processes are difficult to transfer to manufacturing.

Sartobind^® membrane adsorbers have been developed by Sartorius to overcome these challenges. A choice of formats provides the flexibility to purify by centrifugation, pump or FPLC system – and can even eliminate the need for specialist equipment altogether. Pore sizes of 3 – 5 µm reduce diffusion time to support up to 10x faster purification of even the largest proteins and viruses, and high ligand densities provide maximum dynamic binding capacities (DBCs). Furthermore, low bed volumes and negligible dilution reduce buffer requirements by 75% and ensure low-volume, concentrated eluate fractions.

Available in Vivapure^® and Sartobind^® Lab devices, these membrane adsorbers provide research and development scientists with purification solutions that are as simple as filtration. What’s more, the same Sartobind^® platform is utilized in Sartorius process-scale membrane chromatography products, eliminating uncertainty in the transition from research to production.

DHC is an authorized Channel Partner for Sartorius. To get Sartorius products at best discounts mail your enquiry on info@dharidasandco.com